Immunohistochemical study of basement membrane collagen IV in uterine cervix carcinoma
Keywords:
Uterine cervical carcinoma, Basement membrane, Collagen IV, ImmunohistochemistryAbstract
CONTEXT: The integrity of basement membrane (BM) is damaged during the evolution of a benign or potentially malignant lesion into a malignant one, in which it may undergo several degrees of discontinuity as a necessary condition for the invasive process. Immunostaining for collagen IV, which is exclusively found in BM, has been used to evaluate its formation in neoplastic and benign lesions of several organs. OBJECTIVE: To investigate BM continuity pattern in squamous carcinoma "in situ" (CIS), microinvasive (MIC) and invasive (IC) squamous cell carcinoma of the uterine cervix, and to find out if BM expression could be useful in the diagnosis of early stromal invasion (MIC). DESIGN: Archival material between 1988 and 1993 was studied at the Pathological Anatomy Department - Unicamp. PROCEDURES: The selected cases, previously formalin fixed and paraffin embedded, were reviewed retrospectively by submitting them to immunohistochemical study via the avidin-biotin-peroxidase method using a monoclonal antibody anticollagen IV. RESULTS: In all, 17 cases of CIS, 16 of MIC and 21 of IC were evaluated. All IC cases showed evident BM discontinuity, either focal or diffuse. In the CIS group, a continuous BM pattern was predominant, being focally disrupted in only 2/17 cases (11.8%). The MIC group showed an intermediate pattern, but with a clear tendency to BM discontinuity in 10/16 cases (62.5%). Inflammatory infiltrate, a variable also studied, cannot be considered responsible for BM discontinuity, since there was no statistical correlation between them. CONCLUSION: We conclude that immunostaining for collagen IV may contribute to the diagnosis of stromal invasion by BM discontinuity.
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Yurchenco PD, Schittny JC. Molecular architecture of basement membranes. FASEB J 1990;4:1577-90.
Martinez-Hernandez MD, Amenta SP. The basement membrane in pathology. Lab Invest 1983;656-77.
Richards CJ, Furness PN. Basement membrane continuity in benign, premalignant and malignant epithelial conditions of the uterine cervix. Histopathology 1990;16:47-52.
Liotta LA, Abe SD, Robey PG, Martin GR. Preferential digestion of basement membrane collagen by and enzyme derived from a metastatic murine tumor. Proc Natl Acad Sci 1979;76:2268-75.
Barsky SH, Togo S, Garbisa S, Liotta LA. Type IV collagenase immunoreactivity in invasive breast carcinoma. Lancet 1983a;5:296-7.
Shields SE, Ogilvie DJ, McKinnell RG, Tarin D. Degradation of basement membrane collagens by metaloproteases released by human murine and amphibian tumours. J Pathol 1984;143:193-7.
Kramer RH,Vogel G, Nicolson GL. Solubilization and degradation of subendothelial matrix glycoproteins and proteoglycanes by metastatic tumor cells. J Biol Chem 1982;257:2678-86.
Kramer RH, Vogel G, Kathryn G. Selective degradation of basement membrane macromolecules by metastatic melanoma cells. J Natl Cancer Inst 1984;72:889-97.
Soini Y, Autio-Harmainen H. Synthesis and degradation of basement membranes in benign and malignant salivary gland tumours: a study by in situ hybridization. J Pathol 1993;170:291-6.
Barsky H, Siegal GP, Jannotta F, Liotta LA. Loss of basement membrane components by invasive tumors but not by their benign counterparts. Lab Invest 1983;49:140-7.
Havenith MG, Cleutjens JPM, Beek EC, Vander Liden E, De-Goey AFPM, Bosman FT. Human specific anti-type IV collagen monoclonal antibodies, characterization and immunohistochemical application. Histochemistry 1987;87:123-8.
Foelmer HG, Madri JA, Furthmayr H. Methods in laboratory investigation: monoclonal antibodies to type IV collagen: probes for study of structure and function of basement membranes. Lab Invest 1983;48:639-49.
Barsky SH, Baker A, Siegal GP, Togo S, Liotta LA. Use of anti-basement membrane antibodies to distinguish blood vessel capillaries from lymphatic capillaries. Am J Surg Pathol 1983;7:667-77.
Albrechtsen R, Nielsen M, Wewer U, Engvall E, Ruoslahti E. Basement membrane changes in breast cancer detected by immunohistochemical staining for laminin. Cancer Res 1981;41:5076-81.
Liotta LA, Tryggvason K, Garbisa S, Hart I, Foltz CM, Shafie S. Metastatic potential correlates with enzymatic degradation of basement membrane collagen. Nature 1980;284:67-8.
Birembaut P, Caron Y, Adnet JJ, Foidart JM. Usefulness of basement membrane markers in tumoural pathology. J Pathol 1985;145:283-96.
Bosman FT, Havenith M, Cleutjens JPM. Basement membranes in cancer. Ultrastructural Pathol 1985;8:291-304.
Lopes de Faria J, Milani VP, Albino Filho J, Moreira Filho DJC. Geographic pathology and epidemiology of cancer in Brazil (South America). In: Grundmann E, editor. Cancer Epidemiology (1982) Cancer Campaign, vol.6. New York: Gustav Fischer Verlag Stuttgart 1982;71-87.
Figo news: modifications in the staging for stage I vulvar and stage I cervical cancer. Int J Gynecol Obst 1995;50:215-6.
Frappart L, Berger G, Grimaud JA, et al. Basement membrane of the uterine cervix: immunofluorescence characteristics of the collagen epithelium and invasive carcinoma. Gynecol Oncol 1982;13:58-66.
Stenbäck F, Wasenius VM, Ristelli J, Ristelli L. Basement membrane in progressing intraepithelial cervical neoplasia: an ultrastructural and immunohistochemical study with antibodies against human type IV collagen and laminin. Gynaecol Obstet Invest 1985;20:158-66.
Favret M, Parolini S, Rosa D, et al. Localizzazione immunoistochimica di laminina, collagene IV e fibronectina nelle displasie e neoplasie della cervice uterina. Pathologica 1990;82:479-86.
Pitt MA, Hall RJ, Bucklly CH. The distribution of type IV collagen in invasive carcinoma of the uterine cervix. Histopathology 1992;20:139-43.
Uitto VT, Schwartz D, Vers A. Degradation of basement membrane collagen by neutral proteases from human granulocytes. Eur J Biochem 1980;105:409-17.
Li YY, Cheung HT. Basement membrane and its components on lymphocyte adhesion, migration and proliferation. J Immunol 1992;149:3174-81.
Hsu SM, Raine L, Fanger H. A comparative study of the peroxidase-antiperoxidase method and avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibodies. Am J Clin Pathol 1981;75:734-8.
Cattoretti G, Suurmeijer JH. Antigen unmasking on formalin-fixed paraffin-embedded tissues using microwaves: a review. Adv Anat Pathol 1995;2:2-9.
Ozzello L. The behavior of basement membranes in intraductal carcinoma of the breast. Am J Pathol 1959;35:887-99.
Barsky SH, Rao NC, Restrepo C, Liotta LA. Immunocytochemical enhancement of basement membrane antigens by pepsin: applications in diagnostic pathology. Am J Clin Pathol 1984;82:191-4.
Lee AK. Basement membrane and endothelial antigens: their role in evaluation of tumor invasion and metastasis. In: De-Lellis RA, editor. Advances in immunohistochemistry. New York: Raven Press 1988;363-93.
Ehrmann RL, Dwyer IM, Yavner D, Hancock WW. An immunoperoxidase study of laminin and type IV collagen distribution in carcinoma of the cervix and vulva. Obstet Gynecol 1988;72:257-62.
Visser R, Van Der Beek JMH, Havenith MG, Cleutjens JPM, Bosman FT. Immunocytochemical detection of basement membrane antigens in the histopathological evaluation of laryngeal dysplasia and neoplasia. Histopathology 1986;10:171-80.
