Different effects of heating and freezing treatments on the antioxidant properties of broccoli, cauliflower, garlic and onion
an experimental in vitro study
Keywords:
Brassica, Garlic, Onions, AntioxidantsAbstract
BACKGROUND: Vegetables have some beneficial effects on human health due to their antioxidant com-pounds, like polyphenols. Cooking leads to many physical and chemical changes to plant structure that can alter the phytochemical compounds of vegetables. OBJECTIVES: To investigate the effects of heat treatment and freezing on the antioxidant properties of garlic, onion, broccoli and cauliflower. DESIGN AND SETTING: Experimental in vitro study in a university laboratory. METHODS: Fresh broccoli (Brassica oleracea var. italica), cauliflower (Brassica oleracea var. botrytis), garlic (Allium sativum) and onion (Allium cepa) were obtained from a local store. These vegetables were divided into three treatment groups: raw, heated and frozen. The heat treatment consisted of heating them in a drying oven at 150 °C for 20 minutes. The freezing treatment consisted of keeping them frozen at -20 °C until analysis. The total phenolic content, antioxidant activity and malondialdehyde levels of the vegeta-bles were measured using the Folin-Ciocalteu phenol reagent, 2,2-diphenyl-1-picrylhydrazyl radical scav-enging activity and thiobarbituric acid reactive substances, respectively. RESULTS: Heat treatment had deleterious effects on the antioxidant properties of onion and garlic; and it decreased the antioxidant activity of broccoli. Freezing improved the antioxidant activity of broccoli and garlic, but had detrimental effects for cauliflower and onion. CONCLUSIONS: Heat treatment and freezing exhibit different effects on the antioxidant properties of broccoli, cauliflower, garlic and onion. Convenient cooking and storage patterns should be identified for each vegetable, to obtain the best nutritional benefit from the antioxidant compounds of vegetables.
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References
Boeing H, Bechthold A, Bub A, et al. Critical review: vegetables and fruit in the prevention of chronic diseases. Eur J Nutr. 2012;51(6):637-63. PMID: 22684631; doi: 10.1007/s00394-012-0380-y.
Pandey KB, Rizvi SI. Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev. 2009;2(5):270-8. PMID: 20716914; doi: 10.4161/oxim.2.5.9498.
Podsędek A. Natural antioxidants and antioxidant capacity of Brassica vegetables: A review. LWT-Food Science and Technology. 2007;40(1):1-11. doi: 10.1016/j.lwt.2005.07.023.
Latté KP, Appel KE, Lampen A. Health benefits and possible risks of broccoli - An overview. Food Chem Toxicol. 2011;49(12):3287-309. PMID: 21906651; doi: 10.1016/j.fct.2011.08.019.
Hodges DM, Munro KD, Forney CF, Mcrae KB. Glucosinolate and free sugar content in cauliflower (Brassica oleracea var. botrytis cv. Freemont) during controlled-atmosphere storage. Postharvest Biology and Technology. 2006;40(2):123-32. doi: 10.1016/j.postharvbio.2005.12.019.
Llorach R, Espín JC, Tomás-Barberán FA, Ferreres F. Valorization of cauliflower (Brassica oleracea L. var. botrytis) by-products as a source of antioxidant phenolics. J Agric Food Chem. 2003;51(8):2181-7. PMID: 12670153; doi: 10.1021/jf021056a.
Rivlin RS. Historical perspective on the use of garlic. J Nutr. 2001;131(3s):951S-4S. PMID: 11238795; doi: 10.1093/jn/131.3.951S.
Martins NS. Petropoulos IC. Ferreira, Chemical composition and bioactive compounds of garlic (Allium sativum L.) as affected by pre-and post-harvest conditions: A review. Food Chem. 2016.211:41-50. PMID: 27283605; doi: 10.1016/j.foodchem.2016.05.029.
Griffiths G, Trueman L, Crowther T, Thomas B, Smith B. Onions - A global benefit to health. Phytother Res. 2002;16(7):603-15. PMID: 12410539; doi: 10.1002/ptr.1222.
Palermo M, Pellegrini N, FoglianoV. The effect of cooking on the phytochemical content of vegetables. J Sci Food Agric. 2014;94(6):1057-70. PMID: 24227349; doi: 10.1002/jsfa.6478.
Murador D, Braga AR, Da Cunha D, De Rosso V. Alterations in phenolic compound levels and antioxidant activity in response to cooking technique effects: A meta-analytic investigation. Crit Rev Food Sci Nutr. 2018.58(2):169-77. PMID: 26858038; doi: 10.1080/10408398.2016.1140121.
Nicoli MC, Anese M, Parpinel M. Influence of processing on the antioxidant properties of fruit and vegetables. Trends in Food Science & Technology. 1999;10(3):94-100. doi: 10.1016/S0924-2244(99)00023-0.
Giannakourou MC, Taoukis PS. Kinetic modelling of vitamin C loss in frozen green vegetables under variable storage conditions. Food Chem. 2003;83(1):33-41. doi: 10.1016/S0308-8146(03)00033-5.
Dahle LK, Hill EG, Holman RT. The thiobarbituric acid reaction and the autoxidations of polyunsaturated fatty acid methyl esters. Arch Biochem Biophys. 1962;98(2):253-61. doi: 10.1016/0003-9861(62)90181-9.
Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology. 1995;28(1):25-30. doi: 10.1016/S0023-6438(95)80008-5.
Chrzczanowicz J, Gawron A, Zwolinska A, et al. Simple method for determining human serum 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging activity - possible application in clinical studies on dietary antioxidants. Clin Chem Lab Med. 2008;46(3):342-9. PMID: 18254708; doi: 10.1515/CCLM.2008.062.
Obanda M, Owuor PO, Taylor SJ. Flavanol composition and caffeine content of green leaf as quality potential indicators of Kenyan black teas. J Sci Food Agric. 1997; 74(2):209-15. doi: 10.1002/(SICI)1097-0010(199706)74:2<209::AID-JSFA789>3.0.CO;2-4.
Pellegrini N, Chiavaro E, Gardana C, et al. Effect of different cooking methods on color, phytochemical concentration, and antioxidant capacity of raw and frozen brassica vegetables. J Agric Food Chem. 2010;58(7):4310-21. PMID: 20218674; doi: 10.1021/jf904306r.
Zhang D, Hamauzu Y. Phenolics, ascorbic acid, carotenoids and antioxidant activity of broccoli and their changes during conventional and microwave cooking. Food Chem. 2004;88(4):503-9. doi: 10.1016/j. foodchem.2004.01.065.
Bernhardt S, Schlich E. Impact of different cooking methods on food quality: Retention of lipophilic vitamins in fresh and frozen vegetables. J Food Engin. 2006;77(2):327-33. doi: 10.1016/j.jfoodeng.2005.06.040.
Volden J, Borge GI, Hansen M, Bengtsson GB, Wicklund T. Processing (blanching, boiling, steaming) effects on the content of glucosinolates and antioxidant-related parameters in cauliflower (Brassica oleracea L. ssp botrytis). LWT-Food Science and Technology. 2009;42(1):63-73. doi: 10.1016/j.lwt.2008.05.018.
dos Reis LCR, de Oliveira VR, Hagen MEK, et al. Carotenoids, flavonoids, chlorophylls, phenolic compounds and antioxidant activity in fresh and cooked broccoli (Brassica oleracea var. Avenger) and cauliflower (Brassica oleracea var. Alphina F1). LWT-Food Science and Technology. 2015;63(1):177-83. doi: 10.1016/j.lwt.2015.03.089.
Girgin N, El Nehir S. Effects of cooking on in vitro sinigrin bioaccessibility, total phenols, antioxidant and antimutagenic activity of cauliflower (Brassica oleraceae L. var. Botrytis). J Food Composit Anal. 2015;37:119-27. doi: 10.1016/j.jfca.2014.04.013.
Park JH, Park YK, Park E. Antioxidative and antigenotoxic effects of garlic (Allium sativum L.) prepared by different processing methods. Plant Foods Hum Nutr. 2009;64(4):244-9. PMID: 19711184; doi: 10.1007/ s11130-009-0132-1.
Ioku K, Aoyama Y, Tokuno A, et al. Various cooking methods and the flavonoid content in onion. J Nutr Sci Vitaminol (Tokyo). 2001;47(1):78-83. PMID: 11349895; doi: 10.3177/jnsv.47.78.
Rohn S, Buchner N, Driemel G, Rauser M, Kroh LW. Thermal degradation of onion quercetin glucosides under roasting conditions. J Agric Food Chem. 2007;55(4):1568-73. PMID: 17263552; doi: 10.1021/jf063221i.
Lee SU, Lee JH, Choi SH, et al. Flavonoid content in fresh, home-processed, and light-exposed onions and in dehydrated commercial onion products. J Agric Food Chem. 2008;56(18):8541-8. PMID: 18759442; doi: 10.1021/jf801009p.
Harris S, Brunton N, Tiwari U, Cummins E. Human exposure modelling of quercetin in onions (Allium cepa L.) following thermal processing.Food Chem. 2015;187:135-9. PMID: 25977008; doi: 10.1016/j.foodchem.2015.04.035.
Li L, Pegg RB, Eitenmillera RR, Chun JY, Kerrihardc AL. Selected nutrient analyses of fresh, fresh-stored, and frozen fruits and vegetables. J Food Composit Anal. 2017;9(4):8-17. doi: 10.1016/j.jfca.2017.02.002.
Patras A, Tiwari BK, Brunton NP. Influence of blanching and low temperature preservation strategies on antioxidant activity and phytochemical content of carrots, green beans and broccoli. LWT-Food Science and Technology. 2011;44(1):299-306. doi: 10.1016/j.lwt.2010.06.019.
Alanís-Garza PA, Becerra-Moreno A, Mora-Nieves JL, Mora-Mora JP, Jacobo-Velázquez DA. Effect of industrial freezing on the stability of chemopreventive compounds in broccoli. Int J Food Sci Nutr. 2015;66(3):282-88. PMID: 25690928; doi: 10.3109/09637486.2015.1007451.
Murcia MA, Jiménez AM, Martínez-Tomé M. Vegetables antioxidant losses during industrial processing and refrigerated storage. Food Res Int. 2009;42(8):1046-52. doi: 10.1016/j.foodres.2009.04.012.
Volden J, Bengtsson GB, Wicklund T. Glucosinolates, L-ascorbic acid, total phenols, anthocyanins, antioxidant capacities and colour in cauliflower (Brassica oleracea L. ssp botrytis); effects of long-term freezer storage. Food Chem. 2009;112(4):967-76. doi: 10.1016/j. foodchem.2008.07.018.
Ninfali P, Bacchiocca M. Polyphenols and antioxidant capacity of vegetables under fresh and frozen conditions. J Agric Food Chem. 2003;51(8):2222-6. PMID: 12670160; doi: 10.1021/jf020936m.
Miglio C, Chiavaro E, Visconti A, Fogliano V, Pellegrini N. Effects of different cooking methods on nutritional and physicochemical characteristics of selected vegetables. J Agric Food Chem. 2008;56(1):139-47. PMID: 18069785; doi: 10.1021/jf072304b.
