Presence of Mycobacterium avium subsp. paratuberculosis (MAP) in Brazilian patients with inflammatory bowel diseases and in controls

Authors

  • Isabel Azevedo Carvalho Universidade Federal de Viçosa
  • David Germano Gonçalves Schwarz Universidade Federal de Viçosa
  • Pricila Aparecida Grasse Pietralonga Universidade Federal de Viçosa
  • Ana Carolina Silva Faria Universidade Federal de Viçosa
  • Isis Freitas Espechit Braga Universidade Federal de Viçosa
  • Gabriel Domingos Carvalho Instituto Federal do Norte de Minas
  • Fabrício Luciani Valente Universidade Federal de Viçosa
  • João Paulo Machado Universidade Federal de Viçosa
  • Lize Maciel Pinheiro Guimarães Universidade Federal de Minas Gerais
  • Maria de Lourdes Abreu Ferrari Universidade Federal de Minas Gerais
  • Abelardo Silva Júnior Universidade Federal de Viçosa
  • Maria Aparecida Scatamburlo Moreira Universidade Federal de Viçosa

Keywords:

Mycobacterium avium subsp. paratuberculosis, Crohn disease, Inflammatory bowel diseases, Colitis, ulcerative, Brazil

Abstract

CONTEXT AND OBJECTIVE: Mycobacterium avium subsp. paratuberculosis (MAP) has attracted the inter- est of researchers because of similarities between paratuberculosis and Crohn’s disease (CD). The aim of this study was to evaluate the frequency of MAP through cultures, histology and polymerase chain re- action (PCR) on intestinal biopsies from Brazilian CD patients. Quantitative real time PCR (qRT-PCR) was performed on positive samples. DESIGN AND SETTING: Analytical cross-sectional study with control group at two federal universities. METHODS: Fresh samples were collected from 25 patients; five with CD, eight with ulcerative colitis (UC) and 12 controls with non-inflammatory bowel disease (nIBD). Formalin-fixed paraffin-embedded (FFPE) samples from 143 patients were also collected: 44 CD, 49 UC and 56 nIBD. RESULTS: None of the fresh samples was positive for MAP. Five FFPE samples (one CD, two UC and two nIBD) and three fresh samples (one in each group) were positive through IS900-PCR. qRT-PCR was per- formed on these eight samples. Among the FFPE samples, there were 192.12 copies/μl in the CD group, 72.28 copies/μl in UC and 81.43 copies/μl in nIBD. Among the fresh samples, there were 432.99 copies/μl, 167.92 copies/μl and 249.73 copies/μl in the CD, UC and nIBD groups, respectively. The highest bacterial load was in the CD group. CONCLUSION: This study does not provide evidence for a role of MAP in the etiology of CD, although MAP DNA was detected in all three patient groups. This is the first report of MAP presence in human intestinal biopsies in Brazil.

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Author Biographies

Isabel Azevedo Carvalho, Universidade Federal de Viçosa

DVM, PhD. Collaborator, Veterinary Department, Universidade Federal de Viçosa (UFV), Minas Gerais, Brazil.

David Germano Gonçalves Schwarz, Universidade Federal de Viçosa

DVM. Doctoral Student, Veterinary Department, Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brazil.

Pricila Aparecida Grasse Pietralonga, Universidade Federal de Viçosa

DVM, MSc. Collaborator, Veterinary Department, Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brazil.

Ana Carolina Silva Faria, Universidade Federal de Viçosa

DVM. Master’s Student, Veterinary Department, Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brazil.

Isis Freitas Espechit Braga, Universidade Federal de Viçosa

DVM. Doctoral Student, Veterinary Department, Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brazil.

Gabriel Domingos Carvalho, Instituto Federal do Norte de Minas

DVM, PhD. Professor, Veterinary Department, Instituto Federal do Norte de Minas (IFNMG), Salinas, Minas Gerais, Brazil.

Fabrício Luciani Valente, Universidade Federal de Viçosa

DVM, PhD. Postdoctoral Student, Veterinary Department, Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brazil.

João Paulo Machado, Universidade Federal de Viçosa

DVM, PhD. Collaborator, Veterinary Department, Universidade Federal de Viçosa (UFV), Minas Gerais, Brazil.

Lize Maciel Pinheiro Guimarães, Universidade Federal de Minas Gerais

MD. Resident Physician, Medical School, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.

Maria de Lourdes Abreu Ferrari, Universidade Federal de Minas Gerais

MD, PhD. Professor, Medical School, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.

Abelardo Silva Júnior, Universidade Federal de Viçosa

DVM, PhD. Professor, Veterinary Department, Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brazil.

Maria Aparecida Scatamburlo Moreira, Universidade Federal de Viçosa

DVM, PhD. Professor, Veterinary Department, Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brazil.

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Published

2016-02-04

How to Cite

1.
Carvalho IA, Schwarz DGG, Pietralonga PAG, Faria ACS, Braga IFE, Carvalho GD, Valente FL, Machado JP, Guimarães LMP, Ferrari M de LA, Silva Júnior A, Moreira MAS. Presence of Mycobacterium avium subsp. paratuberculosis (MAP) in Brazilian patients with inflammatory bowel diseases and in controls. Sao Paulo Med J [Internet]. 2016 Feb. 4 [cited 2025 Mar. 9];134(1):13-9. Available from: https://periodicosapm.emnuvens.com.br/spmj/article/view/974

Issue

Vol. 134 No. 1 (2016)

Section

Original Article

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