Direct immunofluorescence in Lupus Erythematosus (LE)

Authors

  • Roseli Svartman Isfer University of São Paulo
  • José Antônio Sanches Jr. University of São Paulo
  • Cyro Festa Neto University of São Paulo
  • Selma Schuartz Cernea University of São Paulo
  • Zilda Najjar Prado de Oliveira University of São Paulo
  • Maria Apparecida Constantino Vilela University of São Paulo
  • Maria Luiza Silva University of São Paulo
  • Lucy Massaco Miyachi University of São Paulo
  • Ligia Maria Ishimura Fukumori University of São Paulo
  • Soraia Regina da Silva University of São Paulo

Keywords:

Direct immunofluorescence (DIF), Lupus erythematosus (LE), Discoid lupus erythematosus (DLE), Subacute lupus erythematosus (SALE), Systemic lupus erythematosus (SLE), Immunoglobulins (Igs)

Abstract

One hundred and twenty-six patients with LE were studied. They were distributed as follows: 84 with DLE, 13 with SALE and 29 with SLE. Biopsies from the skin lesions were performed and submitted to DIE Positive results were equal to 69, 61.5 and 72.4 percent of the DLE, SALE and SLE cases, respectively. These data are in accordance with the literature. IgM was the most frequently found immunoglobulin, followed by the association IgM+C3.

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Author Biographies

Roseli Svartman Isfer, University of São Paulo

Universidade de São Paulo, Medical School, General Hospital, São Paulo, São Paulo, Brazil.

José Antônio Sanches Jr., University of São Paulo

Universidade de São Paulo, Medical School, General Hospital, São Paulo, São Paulo, Brazil.

Cyro Festa Neto, University of São Paulo

Universidade de São Paulo, Medical School, General Hospital, São Paulo, São Paulo, Brazil.

Selma Schuartz Cernea, University of São Paulo

Universidade de São Paulo, Medical School, General Hospital, São Paulo, São Paulo, Brazil.

Zilda Najjar Prado de Oliveira, University of São Paulo

Universidade de São Paulo, Medical School, General Hospital, São Paulo, São Paulo, Brazil.

Maria Apparecida Constantino Vilela, University of São Paulo

Universidade de São Paulo, Medical School, General Hospital, São Paulo, São Paulo, Brazil.

Maria Luiza Silva, University of São Paulo

Universidade de São Paulo, Medical School, General Hospital, São Paulo, São Paulo, Brazil.

Lucy Massaco Miyachi, University of São Paulo

Universidade de São Paulo, Medical School, General Hospital, São Paulo, São Paulo, Brazil.

Ligia Maria Ishimura Fukumori, University of São Paulo

Universidade de São Paulo, Medical School, General Hospital, São Paulo, São Paulo, Brazil.

Soraia Regina da Silva, University of São Paulo

Universidade de São Paulo, Medical School, General Hospital, São Paulo, São Paulo, Brazil.

References

Ahmed AR, Provost TT. Incidence of a positive lupus band test using sun-exposed and unexposed skin. Arch Dermatol 1979;115:228-9.

Baart F, Kuyper EH, Cormane RH. The occurrence of certain serum factors in the dermal-epidermal junction and vessel walls of the skin in lupus erythematosus and other skin diseases. Acta Dermatovener 1968;48:578-88.

Blaszczyk M, Dahl MV. Usefulness of direct immunofluorescence in patients with lupus erythematosus. Arch Dermatol 1983;119:1010-7.

Burham TK, Neblett TR, Fine G. The application of the fluorescent antibody technique to the investigation of lupus erythematosus and various dermatoses. J Invest Dermatol 1963;41:451-6.

Cormane RH. "Bound" globulin in the skin of patients with chronic discoid lupus erythematosus and systemic lupus erythematosus. Lancet 1964;1:534-5.

Harrist TJ, Mihm MC. The specificity and clinical usefulness of the lupus band test. Arthritis Rheum 1980;23(4):479-90.

Jordon RE, Schroeter AL, Winkelman RK. Dermal-epidermal deposition of complement components and properdin in systemic lupus erythematosus. Brit J Dermatol 1975;92:263-71.

Monroe EW. Lupus band test. Arch Dermatol 1977;113:830-4.

Nieboer C, Tak-Diamand Z, Van Leeuwen-Wallau HE. Dust-like particles: a specific direct immunofluorescence pattern in sub-acute cutaneous lupus erythematosus. Brit J Dermatol 1988;118:725-34.

Proença NG, Machado ER, Paes RP. Bernardes MP. Reavaliação dos resultados obtidos com a técnica de imunofluorescência direta em lesão de lupus eritematoso discóide. An Bras Dermatol 1985;60:303-6.

Provost TT. Lupus band test. Int J Dermatol 1981;20:475-81.

Ratnam KV, Phay KL, Ng SK, Tan T Skin immunofluorescence patterns in SLE patients in Singapore. Singapore Med J 1987;6:517-9.

Sontheimer RD, Thomas JR, Gilliam JN. Subacute cutaneous lupus erythematosus. Arch Dermatol 1979;115:1409-15.

Sugai AS, Gerbase AB, Cernea SS, et al. Cutaneous lupus erythematosus: direct immunofluorescence and epidermal basal membrane study. Int J Dermatol 1992;31(4):260-4.

Tan EM, Choen AS, Fries JF, et al. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthr and Rheum 1975;25:1271.

Ten Have-Opbraek AAW. Demonstration of immunoglobulin and complement in the skin of patients with lupus erythematosus. Acta Dermatovener 1966;46:68-71.

Tuffanelli DL. Cutaneous immunopathology. Recent observations. J Invest Dermatol 1975;65:143-53.

Tuffanelli DL. Clinical cutaneous immunopathology. J C E Dermatology 1978;16:19-39.

Weingand DA. Cutaneous immunofluorescence. Med Clin of North America 1989;73(5):1263-74.

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Published

1996-03-03

How to Cite

1.
Isfer RS, Sanches Jr. JA, Festa Neto C, Cernea SS, Oliveira ZNP de, Vilela MAC, Silva ML, Miyachi LM, Fukumori LMI, Silva SR da. Direct immunofluorescence in Lupus Erythematosus (LE). Sao Paulo Med J [Internet]. 1996 Mar. 3 [cited 2026 Sep. 4];114(2):1141-7. Available from: https://periodicosapm.emnuvens.com.br/spmj/article/view/1889

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Section

Review and Updating