Post-mortem forensic identity testing
application of PCR to the identification of fire victim
Palavras-chave:
Carbonizados, Tipagem de DNA, PCR, Identificação humana, Investigação de paternidadeResumo
CONTEXTO: A análise do DNA tem sido utilizada com sucesso na identificação de carbonizados e de vítimas de grandes acidentes. A análise do DNA necessita de amostras de sangue dos familiares das vítimas como referência. Os familiares normalmente contribuem com a identificação doando uma amostra de sangue. OBJETIVO: Este trabalho descreve o uso da reação em cadeia de polimerase (PCR) para caracterização genética de uma vítima extensamente queimada pelo fogo. TIPO DE ESTUDO: Relato de caso RELATO DE CASO: O DNA foi extraído de sangue de câmara cardíaca e 15 loci (D1S80, ApoB, D17S30, D3S1744, D18S849, D12S1090, FGA, D7S820, D1S533, D9S304, HUMCSF1PO, HUMTPOX, HUMTHO1, amelogenina e HLA-DQA1) foram analisados utilizando-se a técnica da PCR. Os resultados de todos os loci analisados a partir de DNA genômico extraído do corpo carbonizado foram comparados com os resultados obtidos a partir de DNA genômico extraído de sangue dos supostos pais biológicos do corpo, revelando uma compatibilidade genética.
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Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
