Addition of exogenous cytokines in mixed lymphocyte culture for selecting related donors for bone marrow transplantation

Authors

  • Jeane Eliete Laguila Visentainer Universidade Estadual de Campinas
  • Sofia Rocha Lieber Universidade Estadual de Campinas
  • Lígia Beatriz Lopes Persoli Universidade Estadual de Campinas
  • Afonso Celso Vigorito Universidade Estadual de Campinas
  • Francisco José Penteado Aranha Universidade Estadual de Campinas
  • Cármino Antonio de Souza Universidade Estadual de Campinas

Keywords:

Mixed lymphocyte culture, Bone marrow transplantation, Cytokines, Graft-versus-host disease

Abstract

CONTEXT: Mixed lymphocyte culturing has led to conflicting opinions regarding the selection of donors for bone marrow transplantation. The association between a positive mixed lymphocyte culture and the development of graft-versus-host disease (GVHD) is unclear. The use of exogenous cytokines in mixed lymphocyte cultures could be an alternative for increasing the sensitivity of culture tests. OBJECTIVE: To increase the sensitivity of mixed lymphocyte cultures between donor and recipient human leukocyte antigen (HLA) identical siblings, using exogenous cytokines, in order to predict post-transplantation GVHD and/or rejection. TYPE OF STUDY: Prospective study. SETTING: Bone Marrow Transplantation Unit, Universidade Estadual de Campinas. PARTICIPANTS: Seventeen patients with hematological malignancies and their respective donors selected for bone marrow transplantation procedures. PROCEDURES: Standard and modified mixed lymphocyte culturing by cytokine supplementation was carried out using donor and recipient cells typed for HLA. MAIN MEASUREMENTS: Autologous and allogenic responses in mixed lymphocyte cultures after the addition of IL-4 or IL-2. RESULTS: In comparison with the standard method, average responses in the modified mixed lymphocyte cultures increased by a factor of 2.0 using IL-4 (p < 0.001) and 6.4 using IL-2 (p < 0.001), for autologous donor culture responses. For donor-versus-recipient culture responses, the increase was by a factor of 1.9 using IL-4 (p < 0.001) and 4.1 using IL-2 (p < 0.001). For donor-versus-unrelated culture responses, no significant increase was observed using IL-4, and a mean response inhibition of 20% was observed using IL-2 (p < 0.001). Neither of the cytokines produced a significant difference in the unrelated control versus recipient cell responses. CONCLUSION: IL-4 supplementation was the best for increasing the mixed lymphocyte culture sensitivity. However, IL-4 also increased autologous responses, albeit less intensively than IL-2. Thus, with this loss of specificity we believe that it is not worth modifying the traditional mixed lymphocyte culture method, even with IL-4 addition.

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Author Biographies

Jeane Eliete Laguila Visentainer, Universidade Estadual de Campinas

MD. Universidade Estadual de Maringá, Paraná, Brazil.

Sofia Rocha Lieber, Universidade Estadual de Campinas

MD, PhD. Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.

Lígia Beatriz Lopes Persoli, Universidade Estadual de Campinas

MD, PhD. Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.

Afonso Celso Vigorito, Universidade Estadual de Campinas

MD, PhD. Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.

Francisco José Penteado Aranha, Universidade Estadual de Campinas

MD, PhD. Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.

Cármino Antonio de Souza, Universidade Estadual de Campinas

MD, PhD. Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.

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Published

2002-11-11

How to Cite

1.
Visentainer JEL, Lieber SR, Persoli LBL, Vigorito AC, Aranha FJP, Souza CA de. Addition of exogenous cytokines in mixed lymphocyte culture for selecting related donors for bone marrow transplantation. Sao Paulo Med J [Internet]. 2002 Nov. 11 [cited 2025 Mar. 18];120(6):175-9. Available from: https://periodicosapm.emnuvens.com.br/spmj/article/view/2728

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