Semen analysis in fertile patients undergoing vasectomy
reference values and variations according to age, length of sexual abstinence, seasonality, smoking habits and caffeine intake
Keywords:
Semen, Age groups, Sexual abstinence, Smoking, CaffeineAbstract
CONTEXT AND OBJECTIVE: Recent studies have shown regional and population differences in semen characteristics. The objective was to establish reference values for semen analysis and to verify the effect that age, length of sexual abstinence, seasonality, smoking habits and coffee consumption have on fertile individuals’ semen characteristics. DESIGN AND SETTING: Prospective study in the Urology Division, Hospital das Clínicas, Universidade de São Paulo. METHODS: Between September 1999 and August 2002, 500 fertile men requesting a vasectomy for sterilization purposes were asked to provide a semen sample before the vasectomy. We evaluated the effects of age, sexual abstinence, seasonality, smoking and coffee consumption on semen characteristics. RESULTS: Compared with World Health Organization values, 87.2% of the patients presented sperm morphology below the normal level. A significant decline in semen volume, sperm motility and sperm morphology in patients over 45 years of age was observed. In patients with 5 days or more of abstinence, there was reduced sperm motility. The lowest values for sperm concentration, motility and morphology were observed in summer and the highest in winter. No differences in semen parameters relating to smoking were detected. Patients who drank six or more cups of coffee per day presented higher sperm motility. CONCLUSIONS: Our sample had a very low percentage of normal sperm morphology. Only sperm morphology showed a high abnormality rate. Differences in semen parameters with regard to age, length of sexual abstinence, seasonality and coffee consumption were identified. No differences relating to smoking were detected.
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References
World Health Organization. WHO laboratory manual for the examination of human semen and semen-cervical mucus interaction. Singapore: Press Concern; 1980.
World Health Organization. WHO laboratory manual for the examination of human semen and semen-cervical mucus interac- tion. 2nd ed. Cambridge: Cambridge University Press; 1987.
World Health Organization. WHO laboratory manual for the examination of human semen and semen-cervical mucus interac- tion. 3rd ed. Cambridge: Cambridge University Press; 1992.
World Health Organization. WHO laboratory manual for the examination of human semen and semen-cervical mucus interac- tion. 4th ed. Cambridge: Cambridge University Press; 1999.
Menkveld R, Wong WY, Lombard CJ, et al. Semen parameters, including WHO and strict criteria morphology, in a fertile and subfertile population: an effort towards standardization of in- vivo thresholds. Hum Reprod. 2001;16(6):1165-71.
Comhaire FH, Huysse S, Hinting A, Vermeulen L, Schoonjans F. Objective semen analysis: has the target been reached? Hum Reprod. 1992;7(2):237-41.
Coetzee K, Kruge TF, Lombard CJ. Predictive value of normal sperm morphology: a structured literature review. Hum Reprod Update. 1998;4(1):73-82.
Moutel G, Christian-Herve, Adnet JJ. Has traditional sperm analysis lost its clinical relevance? Fertil Steril. 1997;67(3):583-4; author reply 584-5.
Lemcke B, Behre HM, Nieschlag E. Frequently subnormal se- men profiles of normal volunteers recruited over 17 years. Int J Androl. 1997;20(3):144-52.
Spandorfer SD, Avrech OM, Colombero LT, Palermo GD, Ros- enwaks Z. Effect of parental age on fertilization and pregnancy characteristics in couples treated by intracytoplasmic sperm injection. Hum Reprod. 1998;13(2):334-8.
Vine MF. Smoking and male reproduction: a review. Int J Androl. 1996;19(6):323-37.
Andolz P, Bielsa MA, Andolz A. Circannual variation in human semen parameters. Int J Androl. 2001;24(5):266-71.
Jensen TK, Henriksen TB, Hjollund NH, et al. Caffeine intake and fecundability: a follow-up study among 430 Danish couples planning their first pregnancy. Reprod Toxicol. 1998;12(3):289-95.
Carlsen E, Giwercman A, Keiding N, Skakkebaek NE. Evidence for decreasing quality of semen during past 50 years. BMJ. 1992;305(6854):609-13.
Irvine S, Cawood E, Richardson D, MacDonald E, Aitken J. Evidence of deteriorating semen quality in the United Kingdom: birth cohort study in 577 men in Scotland over 11 years. BMJ. 1996;312(7029):467-71.
Jorgensen N, Andersen AG, Eustache F, et al. Regional differences in semen quality in Europe. Hum Reprod. 2001;16(5):1012-9.
Auger J, Jouannet P. Evidence for regional differences of semen quality among fertile French men. Federation Française des Centres d’Étude et de Conservation des Oeufs et du Sperme humains. Hum Reprod. 1997;12(4):740-5.
Tanemura K, Kurohmaru M, Kuramoto K, Hayashi Y. Age- related morphological changes in the testis of the BDF1 mouse. J Vet Med Sci. 1993;55(5):703-10.
Parkening TA, Collins TJ, Au WW. Paternal age and its ef- fects on reproduction in C57BL/6NNia mice. J Gerontol. 1988;43(3):B79-84.
Gray A, Feldman HA, McKinlay JB, Longcope C. Age, disease, and changing sex hormone levels in middle-aged men: results of the Massachusetts Male Aging Study. J Clin Endocrinol Metab. 1991;73(5):1016-25.
Eskenazi B, Wyrobek AJ, Sloter E, et al. The association of age and semen quality in healthy men. Hum Reprod. 2003;18(2):447-54.
Kidd SA, Eskenazi B, Wyrobek AJ. Effects of male age on se- men quality and fertility: a review of the literature. Fertil Steril. 2001;75(2):237-48.
Rolf C, Behre HM, Nieschlag E. Reproductive parameters of older compared to younger men of infertile couples. Int J Androl. 1996;19(3):135-42.
Plas E, Berger P, Hermann M, Pfluger H. Effects of aging on male fertility? Exp Gerontol. 2000;35(5):543-51.
Pellestor F, Girardet A, Andreo B. Effect of long abstinence periods on human sperm quality. Int J Fertil Menopausal Stud. 1994;39(5):278-82.
Mortimer D, Templeton AA, Lenton EA, Coleman RA. Influ- ence of abstinence and ejaculation-to-analysis delay on semen analysis parameters of suspected infertile men. Arch Androl. 1982;8(4):251-6.
Raziel A, Friedler S, Schachter M, et al. Influence of a short or long abstinence period on semen parameters in the ejaculate of patients with nonobstructive azoospermia. Fertil Steril. 2001;76(3):485-90.
Krause A, Krause W. Seasonal variations in human seminal param- eters. Eur J Obstet Gynecol Reprod Biol. 2002;101(2):175-8.
Saint Pol P, Beuscart R, Leroy-Martin B, Hermand E, Jablonski W. Circannual rhythms of sperm parameters of fertile men. Fertil Steril. 1989;51(6):1030-3.
Schalue TK, Webert SK, Rognsvoog LM, et al. Monthly variation among semen samples from 4114 infertility patients. Cincinnati: Annual Meeting of the American Society for Re- productive Medicine; 1997. [abstract].
Centola GM, Eberly S. Seasonal variations and age-related changes in human sperm count, motility, motion parameters, morphology, and white blood cell concentration. Fertil Steril. 1999;72(5):803-8.
Levine RJ, Bordson BL, Mathew RM, Brown MH, Stanley JM, Star TB. Deterioration of semen quality during summer in New Orleans. Fertil Steril. 1988;49(5):900-7.
Politoff L, Birkhauser M, Almendral A, Zorn A. New data confirming a circannual rhythm in spermatogenesis. Fertil Steril. 1989;52(3):486-9.
Snyder PJ. Fewer sperm in the summer - it’s not heat, it’s… N Engl J Med. 1990;323(1):54-6.
Levine RJ, Brown MH, Bell M, Shue F, Greenberg GN, Bordson BL. Air-conditioned environments do not prevent deteriora- tion of human semen quality during the summer. Fertil Steril. 1992;57(5):1075-83.
