Evaluation of three methods for hemoglobin measurement in a blood donor setting

Authors

  • Jacob Rosenblit Hospital Israelita Albert Einstein
  • Cláudia Regina Abreu Hospital Israelita Albert Einstein
  • Leonel Nulman Szterling Hospital Israelita Albert Einstein
  • José Mauro Kutner Hospital Israelita Albert Einstein
  • Nelson Hamerschlak Hospital Israelita Albert Einstein
  • Paula Frutuoso Hospital Israelita Albert Einstein
  • Thelma Regina Silva Stracieri de Paiva Hospital Israelita Albert Einstein
  • Orlando da Costa Ferreira Júnior Hospital Israelita Albert Einstein

Keywords:

Hemoglobin, Methods, Quantification, Equipment

Abstract

CONTEXT: The hemoglobin (Hb) level is the most-used parameter for screening blood donors for the presence of anemia, one of the most-used methods for measuring Hb levels is based on photometric detection of cyanmetahemoglobin, as an alternative to this technology, HemoCue has developed a photometric method based on the determination of azide metahemoglobin. OBJECTIVE: To evaluate the performance of three methods for hemoglobin (Hb) determination in a blood bank setting. DESIGN: Prospective study utilizing blood samples to compare methods for Hb determination. SETTING: Hemotherapy Service of the Hospital Israelita Albert Einstein, a private institution in the tertiary health care system. SAMPLE: Serial blood samples were collected from 259 individuals during the period from March to June 1996. MAIN MEASUREMENTS: Test performances and their comparisons were assessed by the analysis of coefficients of variation (CV), linear regression and mean differences. RESULTS: The CV for the three methods were: Coulter 0.68%, Cobas 0.82% and HemoCue 0.69%. There was no difference between the mean Hb determination for the three methods (p>0.05). The Coulter and Cobas methods showed the best agreement and the HemoCue method gave a lower Hb determination when compared to both the Coulter and Cobas methods. However, pairs of methods involving the HemoCue seem to have narrower limits of agreement (± 0.78 and ± 1.02) than the Coulter and Cobas combination (± 1.13). CONCLUSION: The three methods provide good agreement for hemoglobin determination.

Downloads

Download data is not yet available.

Author Biographies

Jacob Rosenblit, Hospital Israelita Albert Einstein

MD, Hemotherapy Service, Hospital Israelita Albert Einstein.

Cláudia Regina Abreu, Hospital Israelita Albert Einstein

BSC, Hemotherapy Service, Hospital Israelita Albert Einstein.

Leonel Nulman Szterling, Hospital Israelita Albert Einstein

MD, Hemotherapy Service, Hospital Israelita Albert Einstein.

José Mauro Kutner, Hospital Israelita Albert Einstein

MD, Hemotherapy Service, Hospital Israelita Albert Einstein.

Nelson Hamerschlak, Hospital Israelita Albert Einstein

MD, Hemotherapy Service, Hospital Israelita Albert Einstein.

Paula Frutuoso, Hospital Israelita Albert Einstein

BSc, Hemotherapy Service, Hospital Israelita Albert Einstein.

Thelma Regina Silva Stracieri de Paiva, Hospital Israelita Albert Einstein

BSC, Hemotherapy Service, Hospital Israelita Albert Einstein.

Orlando da Costa Ferreira Júnior, Hospital Israelita Albert Einstein

MD, PhD. Hemotherapy Service, Hospital Israelita Albert Einstein.

References

Von Schenck, Falkensson M, Lundberg B. Evaluation of HemoCue, a new Device for Determining Hemoglobin. Clin Chem 1986;32:526-9.

ICSH. Recommendations for Reference Method for Hemoglobinometry in Human Blood (ICSH standard 1995) and Specifications for International Hemoglobincyamide Standard (4th edition). J Clin Pathol 1996;49:271-4.

Nicholls PD. An evaluation of the HemoCue for Correcting the Hemoglobin Value of Lipemic Samples. Med Lab Sciences 1990;47:226-9.

Cable RG. Hemoglobin Determination in Blood Donors. Transfusion Med Ver 1995;9:131-44.

Carlson DA, Daigneault RW, Statland BE. Evaluation of the HemoCue photometer for measurement of blood donor hemoglobin. Abstract for the 40th anniversary meeting of the American Association of Blood Banks, Orlando, FL, 1987.

Dean AG, Dean JA, Burton AH, Dicker RC. Epi Info, version 5: a word processing, database, and statistics program for epidemiology on microcomputers. Stone Mountain, GA: USD; 1990.

Bland JM, Altman DG. Statistical Methods for Assessing Agreement Between two Methods of Clinical Measurement. Lancet 1986;i:307-10.

Downloads

Published

1999-05-05

How to Cite

1.
Rosenblit J, Abreu CR, Szterling LN, Kutner JM, Hamerschlak N, Frutuoso P, Paiva TRSS de, Ferreira Júnior O da C. Evaluation of three methods for hemoglobin measurement in a blood donor setting. Sao Paulo Med J [Internet]. 1999 May 5 [cited 2025 Mar. 18];117(3):108-12. Available from: https://periodicosapm.emnuvens.com.br/spmj/article/view/2505

Issue

Section

Original Article