Increased exhalation of hydrogen peroxide in healthy subjects following cigarette consumption
Keywords:
Hydrogen peroxide, Smoking, Free radicalsAbstract
CONTEXT: Increased hydrogen peroxide has been described in the expired breath condensate (H2O2-E) of several lung conditions, such as acute respiratory distress syndrome, chronic obstructive pulmonary disease and asthma. This technique has been advocated as being a simple method for documenting airway inflammation. OBJECTIVE: To evaluate H2O2-E in healthy cigarette smokers, and to determine the acute effects of the consumption of one cigarette on H2O2-E levels. TYPE OF STUDY: Prospective, controlled trial. SETTING: A pulmonary function laboratory in a University Hospital. PARTICIPANTS: Two groups of healthy volunteers: individuals who had never smoked (NS; n=10; 4 men; age = 30.6 ± 6.2 years) and current cigarette smokers (S; n=12; 7 men; age = 38.7 ± 9.8). None of the volunteers had respiratory symptoms and all showed normal spirometric tests. INTERVENTION: Expired air was collected from all volunteers through a face mask and a plastic collecting system leading into a flask with dry ice and pure ethanol. Samples from the group S were collected twice, before and half an hour after the combustion of one cigarette. MAIN MEASUREMENTS: Expired hydrogen peroxide using the Gallati and Pracht method. RESULTS: The S and NS groups showed comparable levels of H2O2-E at basal conditions [NS = 0.74 muM (DP 0.24) vs. S = 0.75 muM (DP 0.31)]. The smokers showed a significant increase in H2O2-E levels half an hour after the consumption of only one cigarette [0.75 muM (DP 0.31) vs. 0.95 muM (DP 0.22)]. CONCLUSION: The present results are consistent with the concept that smokers increase oxidative stress with elevated production of reactive oxygen species, contributing to the development of smoking-related disorders.
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Cross CE, Halliwell B, Borish ET, Pryor WA, Ames BN, Saul RL, McCord JM, Harman D. Oxygen radicals and human disease. Ann Intern Med 1987;107:526-45.
Rahman I, MacNee W. Role of oxidants/antioxidants in smoking-induced lung diseases. Free Rad Biol Med 1996;21:669-81.
Pryor W, Stone K. Oxidants in cigarette smoke. Radicals, hydrogen peroxide, peroxynitrate and peroxynitrite. Ann N York Acad Sci 1993;686:12-27.
Niki E, Minamisawa S, Oikawa M, Komuro E. Membrane damage from lipid oxidation induced by free radicals and cigarette smoke. Ann N York Acad Sci 1993;686:29-38.
Hyslop PA, Sklar LA. A quantitative fluorometric assay for the determination of oxidant production by polymorphonuclear leukocytes: its use in the simultaneous fluorometric assay of cellular activation processes. Anal Bioch 1984;141:280-6.
Baldwin SR, Grum CM, Boxer LA, Simon RH, Ketai LH, Devall LJ. Oxidant activity in expired breath of patients with adult respiratory distress syndrome. Lancet 1986;1:11-4.
Sznajder JI, Fraiman A, Hall JB, et al. Increased hydrogen peroxide in the expired breath of patients with acute hypoxemic respiratory failure. Chest 1989;96:606-12.
Kietzmann D, Kahl R, Müller M, Burchardi H, Kettler D. Hydrogen peroxides in expired breath condensate of patients with acute respiratory failure and with ARDS. Int Care Med 1993;19:78-81.
Dekhuijzen PNR, Aben KKH, Dekker I, et al. Increased exhalation of hydrogen peroxide in patients with stable and unstable chronic obstructive pulmonary disease. Am J Respir Crit Care Med 1996;154:813-6.
Dohlman AW, Black HR, Royall JA. Expired breath hydrogen peroxide is a marker of acute airway inflammation in pediatric patients with asthma. Am J Respir Crit Care Med 1993;148:955-60.
Gallati H, Pracht I. Horseradish peroxidase: kinetic studies and optimization of peroxidase activity determination using the substrates H2O2 and 3,3˘,5,5˘-tetramethylbenzidine. J Clin Chem Clin Bioch 1985;23:453-60.
Knudson RJ, Slatin RC, Lebowitz MD. The maximal expiratory flow-volume curve: normal standards, variability, and effects of age. Am Rev Respir Dis 1976;113:587-600.
Crapo RO, Morris AH, Gardner RM. Reference spirometric values using techniques and equipment that meet ATS recommendations. Am Rev Respir Dis 1981;123:659-64.
Lundeberg JON, Weitzberg E, Lundberg JM, Alving K. Nitric oxide in exhaled air. Eur Respir J 1996;9:2671-80.
Barnes PJ, Kharitonov SA. Exhaled nitric oxide: a new lung function test. Thorax 1996;51:233-7.
Singh S, Evans TW. Nitric oxide, the biological mediator of the decade: fact or fiction? Eur Respir J 1997;10:699-707.
Williams MD, Chance B. Spontaneous chemiluminescence of human breath. Spectrum, lifetime, temporal distribution, and correlation with peroxide. J Biol Chem 1983;258:3628-31.
Horvath I, Donnely LE, Kiss A, et al. Combined use of exhaled hydrogen peroxide and nitric oxide in monitoring asthma. Am J Respir Crit Care Med 1998;158:1042-6.
Silkoff PE, McClean PA, Caramori M, Slutsky AS, Zamel N. A significant proportion of exhaled nitric oxide arises in large airways in normal subjects. Respir Physiol 1998;113:33-8.
MacNee W, Wiggs B, Belzberg AS, Hogg JC. The effect of cigarette smoking on neutrophil kinetics in human lungs. N E J M 1989;321:924-.
Drost EM, Selby C, Bridgeman MME, MacNee W. Decreased leukocyte deformability after acute cigarette smoking in humans. Am Rev Respir Dis 1993;148:1277-83.
Skwarski KM, Gorecka D, Sliwinski P, Hogg JC, MacNee W. The effects of cigarette smoking on pulmonary hemodynamics. Chest 1993;103:166-72.
Nowac D, Antczak A, Krol M, et al. Increased content of hydrogen peroxide in the expired breath of cigarette smokers. Eur Respir J 1996;9:652-7.
